Fabio Piano

Fabio Piano

Professor of Biology; Provost, NYU Abu Dhabi; Founding Director, Center for Genomics and Systems Biology

Ph.D. 1995 (Biology), New York University; M.A. 1991 (Biology), New York University; B.S. 1988 (Biology), New York University.

Office Address: 

New York University
Department of Biology
Center for Genomics and Systems Biology
12 Waverly Place, Room 708
New York, NY 10003

Phone: 

(212) 998-3737

Fax: 

(212) 995-4015

Areas of Research/Interest: 

Genomics, genetics and evolution of early C. elegans development.

List of Publications from Pubmed

List of citations from Google Scholar

Research:

We study the genetic and evolutionary mechanisms underlying early embryonic development using a combination of molecular genetic and functional genomics approaches in the animal model C. elegans and related nematodes. One of our major goals is to use RNA interference (RNAi) of ovary-expressed genes followed by time-lapse microscopy to work toward a comprehensive molecular description of early embryogenesis in C. elegans. RNAi offers a powerful way to obtain information about the loss-of-function phenotype of the genes tested, while the early embryo offers a system in which basic cellular and developmental processes can be easily studied. We currently have tested over 1,000 genes and identified about 300 genes required for embryogenesis. Although most of these genes are highly conserved, fewer than 10% have been identified in previous genetic screens. We use the data obtained from the RNAi tests to build gene clusters based on phenotypic analysis. The clusters are then used to guide two broad lines of investigation: (1) functional analysis of the genome, and (2) molecular dissection of specific cellular processes.

From the initial clustering studies, we have found groups of genes required for basic processes such as nuclear movements, mitotic spindle formation, cytokinesis, cell cycle progression, and proper asymmetric cell division. In many cases, these clusters contain genes that are conserved in humans. However, their function in humans is not yet known. Therefore, our data can be used not only to analyze the C. elegans genome but also to guide the functional examination of the human genome. In our current dataset we have already found genes for which the human homolog is associated with a genetic disease, including some that have been previously targeted for anti-cancer drug development.

In a related project we are using the early nematode embryo as a model to study the evolution of developmental mechanisms. Comparisons across species have revealed fundamental differences during early embryogenesis. Significantly, in some species the wild-type patterns of early cleavages resemble those produced by various C. elegans mutants. We are examining these phenotypic differences in conjunction with molecular analyses to identify mechanisms underlying the phenotypic diversity seen in nature.

Fellowships/Honors:

Post-doctoral fellowship from the Damon Runyon-Walter Winchell Foundation for Cancer Research; the Gladys Mateyko Award for Excellence in Biology; the James Arthur Fellowship.

Selected Works:

Uncovering buffered pleiotropy: a genome-scale screen for mel-28 genetic interactors in Caenorhabditis elegans.
G3 (Bethesda)   (2014 Jan);  PMC3887534 free full-text archive
Fernandez AG, Mis EK, Lai A, Mauro M, Quental A, Bock C, Piano F
 
A compendium of RNA-binding motifs for decoding gene regulation.
Nature   (2013 Jul 11);  PMC3929597 free full-text archive
Ray D, Kazan H, Cook KB, Weirauch MT, Najafabadi HS, Li X, Gueroussov S, Albu M, Zheng H, Yang A, Na H, Irimia M, Matzat LH, Dale RK, Smith SA, Yarosh CA, Kelly SM, Nabet B, Mecenas D, Li W, Laishram RS, Qiao M, Lipshitz HD, Piano F, Corbett AH, Carstens RP, Frey BJ, Anderson RA, Lynch KW, Penalva LO, Lei EP, Fraser AG, Blencowe BJ, Morris QD, Hughes TR
 
DevStaR: high-throughput quantification of C. elegans developmental stages.
IEEE Trans Med Imaging   (2013 Oct);   PMID: 23722463
White AG, Lees B, Kao HL, Cipriani PG, Munarriz E, Paaby AB, Erickson K, Guzman S, Rattanakorn K, Sontag E, Geiger D, Gunsalus KC, Piano F
 
High-throughput fluorescence-based isolation of live C. elegans larvae.
Nat Protoc   (2012 Aug);   PMID: 22814389
Fernandez AG, Bargmann BO, Mis EK, Edgley ML, Birnbaum KD, Piano F
 
RNAi methods and screening: RNAi based high-throughput genetic interaction screening.
Methods Cell Biol   (2011);   PMID: 22118275
Cipriani PG, Piano F
 
A model of cytoplasmically driven microtubule-based motion in the single-celled Caenorhabditis elegans embryo.
Proc Natl Acad Sci U S A   (2011 Jun 28);  PMC3127902 free full-text archive
Shinar T, Mana M, Piano F, Shelley MJ
 
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.
Cell   (2011 Apr 29);  PMC3086541 free full-text archive
Green RA, Kao HL, Audhya A, Arur S, Mayers JR, Fridolfsson HN, Schulman M, Schloissnig S, Niessen S, Laband K, Wang S, Starr DA, Hyman AA, Schedl T, Desai A, Piano F, Gunsalus KC, Oegema K
 
Integrative analysis of the Caenorhabditis elegans genome by the modENCODE project.
Science   (2010 Dec 24);  PMC3142569 free full-text archive
Gerstein MB, Lu ZJ, Van Nostrand EL, Cheng C, Arshinoff BI, Liu T, Yip KY, Robilotto R, Rechtsteiner A, Ikegami K, Alves P, Chateigner A, Perry M, Morris M, Auerbach RK, Feng X, Leng J, Vielle A, Niu W, Rhrissorrakrai K, Agarwal A, Alexander RP, Barber G, Brdlik CM, Brennan J, Brouillet JJ, Carr A, Cheung MS, Clawson H, Contrino S, Dannenberg LO, Dernburg AF, Desai A, Dick L, Dose AC, Du J, Egelhofer T, Ercan S, Euskirchen G, Ewing B, Feingold EA, Gassmann R, Good PJ, Green P, Gullier F, Gutwein M, Guyer MS, Habegger L, Han T, Henikoff JG, Henz SR, Hinrichs A, Holster H, Hyman T, Iniguez AL, Janette J, Jensen M, Kato M, Kent WJ, Kephart E, Khivansara V, Khurana E, Kim JK, Kolasinska-Zwierz P, Lai EC, Latorre I, Leahey A, Lewis S, Lloyd P, Lochovsky L, Lowdon RF, Lubling Y, Lyne R, MacCoss M, Mackowiak SD, Mangone M, McKay S, Mecenas D, Merrihew G, Miller DM 3rd, Muroyama A, Murray JI, Ooi SL, Pham H, Phippen T, Preston EA, Rajewsky N, Ratsch G, Rosenbaum H, Rozowsky J, Rutherford K, Ruzanov P, Sarov M, Sasidharan R, Sboner A, Scheid P, Segal E, Shin H, Shou C, Slack FJ, Slightam C, Smith R, Spencer WC, Stinson EO, Taing S, Takasaki T, Vafeados D, Voronina K, Wang G, Washington NL, Whittle CM, Wu B, Yan KK, Zeller G, Zha Z, Zhong M, Zhou X, Ahringer J, Strome S, Gunsalus KC, Micklem G, Liu XS, Reinke V, Kim SK, Hillier LW, Henikoff S, Piano F, Snyder M, Stein L, Lieb JD, Waterston RH
 
Rapid and accurate developmental stage recognition of C. elegans from high-throughput image data.
Proc IEEE Comput Soc Conf Comput Vis Pattern Recognit   (2010 Aug 5);  PMC3205469 free full-text archive
White AG, Cipriani PG, Kao HL, Lees B, Geiger D, Sontag E, Gunsalus KC, Piano F
 
The landscape of C. elegans 3'UTRs.
Science   (2010 Jul 23);  PMC3142571 free full-text archive
Mangone M, Manoharan AP, Thierry-Mieg D, Thierry-Mieg J, Han T, Mackowiak SD, Mis E, Zegar C, Gutwein MR, Khivansara V, Attie O, Chen K, Salehi-Ashtiani K, Vidal M, Harkins TT, Bouffard P, Suzuki Y, Sugano S, Kohara Y, Rajewsky N, Piano F, Gunsalus KC, Kim JK
 
Automated sorting of live C. elegans using laFACS.
Nat Methods   (2010 Jun);  PMC2896029 free full-text archive
Fernandez AG, Mis EK, Bargmann BO, Birnbaum KD, Piano F
 
EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans.
Curr Biol   (2009 Nov 3);  PMC2783797 free full-text archive
Parry JM, Velarde NV, Lefkovith AJ, Zegarek MH, Hang JS, Ohm J, Klancer R, Maruyama R, Druzhinina MK, Grant BD, Piano F, Singson A
 
Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression.
Nat Methods   (2009 Oct);  PMC2756031 free full-text archive
Stoeckius M, Maaskola J, Colombo T, Rahn HP, Friedlander MR, Li N, Chen W, Piano F, Rajewsky N
 
Evolution of early embryogenesis in rhabditid nematodes.
Dev Biol   (2009 Nov 1);  PMC2763944 free full-text archive
Brauchle M, Kiontke K, MacMenamin P, Fitch DH, Piano F
 
Unlocking the secrets of the genome.
Nature   (2009 Jun 18);  PMC2843545 free full-text archive
Celniker SE, Dillon LA, Gerstein MB, Gunsalus KC, Henikoff S, Karpen GH, Kellis M, Lai EC, Lieb JD, MacAlpine DM, Micklem G, Piano F, Snyder M, Stein L, White KP, Waterston RH
 
A protein domain-based interactome network for C. elegans early embryogenesis.
Cell   (2008 Aug 8);  PMC2596478 free full-text archive
Boxem M, Maliga Z, Klitgord N, Li N, Lemmens I, Mana M, de Lichtervelde L, Mul JD, van de Peut D, Devos M, Simonis N, Yildirim MA, Cokol M, Kao HL, de Smet AS, Wang H, Schlaitz AL, Hao T, Milstein S, Fan C, Tipsword M, Drew K, Galli M, Rhrissorrakrai K, Drechsel D, Koller D, Roth FP, Iakoucheva LM, Dunker AK, Bonneau R, Gunsalus KC, Hill DE, Piano F, Tavernier J, van den Heuvel S, Hyman AA, Vidal M
 
Diverse roles of actin in C. elegans early embryogenesis.
BMC Dev Biol   (2007);  PMC2323969 free full-text archive
Velarde N, Gunsalus KC, Piano F
 
C. elegans network biology: a beginning.
WormBook   (2006);   PMID: 18050437
Piano F, Gunsalus KC, Hill DE, Vidal M
 
UTRome.org: a platform for 3'UTR biology in C. elegans.
Nucleic Acids Res   (2008 Jan);  PMC2238901 free full-text archive
Mangone M, Macmenamin P, Zegar C, Piano F, Gunsalus KC
 
EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans.
Curr Biol   (2007 Sep 18);   PMID: 17869112
Maruyama R, Velarde NV, Klancer R, Gordon S, Kadandale P, Parry JM, Hang JS, Rubin J, Stewart-Michaelis A, Schweinsberg P, Grant BD, Piano F, Sugimoto A, Singson A
 
Forward chemical genetic approach identifies new role for GAPDH in insulin signaling.
Nat Chem Biol   (2007 Jan);   PMID: 17115034
Min J, Kyung Kim Y, Cipriani PG, Kang M, Khersonsky SM, Walsh DP, Lee JY, Niessen S, Yates JR 3rd, Gunsalus K, Piano F, Chang YT
 
MEL-28 is downstream of the Ran cycle and is required for nuclear-envelope function and chromatin maintenance.
Curr Biol   (2006 Sep 5);   PMID: 16950115
Fernandez AG, Piano F
 
A peptidomimetic siRNA transfection reagent for highly effective gene silencing.
Mol Biosyst   (2006 Jun);   PMID: 16880950
Utku Y, Dehan E, Ouerfelli O, Piano F, Zuckermann RN, Pagano M, Kirshenbaum K
 
Two phases of astral microtubule activity during cytokinesis in C. elegans embryos.
Dev Cell   (2006 Apr);   PMID: 16580995
Motegi F, Velarde NV, Piano F, Sugimoto A
 
A genome-wide map of conserved microRNA targets in C. elegans.
Curr Biol   (2006 Mar 7);   PMID: 16458514
Lall S, Grun D, Krek A, Chen K, Wang YL, Dewey CN, Sood P, Colombo T, Bray N, Macmenamin P, Kao HL, Gunsalus KC, Pachter L, Piano F, Rajewsky N
 
Nucleoporins NPP-1, NPP-3, NPP-4, NPP-11 and NPP-13 are required for proper spindle orientation in C. elegans.
Dev Biol   (2006 Jan 15);  PMC1405919 free full-text archive
Schetter A, Askjaer P, Piano F, Mattaj I, Kemphues K
 
Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis.
Mol Biol Cell   (2005 Dec);  PMC1289429 free full-text archive
Lall S, Piano F, Davis RE
 
Toward automatic phenotyping of developing embryos from videos.
IEEE Trans Image Process   (2005 Sep);   PMID: 16190471
Ning F, Delhomme D, LeCun Y, Piano F, Bottou L, Barbano PE
 
Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis.
Nature   (2005 Aug 11);   PMID: 16094371
Gunsalus KC, Ge H, Schetter AJ, Goldberg DS, Han JD, Hao T, Berriz GF, Bertin N, Huang J, Chuang LS, Li N, Mani R, Hyman AA, Sonnichsen B, Echeverri CJ, Roth FP, Vidal M, Piano F
 
New genes with roles in the C. elegans embryo revealed using RNAi of ovary-enriched ORFeome clones.
Genome Res   (2005 Feb);  PMC546526 free full-text archive
Fernandez AG, Gunsalus KC, Huang J, Chuang LS, Ying N, Liang HL, Tang C, Schetter AJ, Zegar C, Rual JF, Hill DE, Reinke V, Vidal M, Piano F
 
RNAi as a tool to study cell biology: building the genome-phenome bridge.
Curr Opin Cell Biol   (2005 Feb);   PMID: 15661512
Gunsalus KC, Piano F
 
Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss.
Proc Natl Acad Sci U S A   (2004 Jun 15);  PMC428462 free full-text archive
Kiontke K, Gavin NP, Raynes Y, Roehrig C, Piano F, Fitch DH
 
A map of the interactome network of the metazoan C. elegans.
Science   (2004 Jan 23);  PMC1698949 free full-text archive
Li S, Armstrong CM, Bertin N, Ge H, Milstein S, Boxem M, Vidalain PO, Han JD, Chesneau A, Hao T, Goldberg DS, Li N, Martinez M, Rual JF, Lamesch P, Xu L, Tewari M, Wong SL, Zhang LV, Berriz GF, Jacotot L, Vaglio P, Reboul J, Hirozane-Kishikawa T, Li Q, Gabel HW, Elewa A, Baumgartner B, Rose DJ, Yu H, Bosak S, Sequerra R, Fraser A, Mango SE, Saxton WM, Strome S, Van Den Heuvel S, Piano F, Vandenhaute J, Sardet C, Gerstein M, Doucette-Stamm L, Gunsalus KC, Harper JW, Cusick ME, Roth FP, Hill DE, Vidal M
 
RNAiDB and PhenoBlast: web tools for genome-wide phenotypic mapping projects.
Nucleic Acids Res   (2004 Jan 1);  PMC308844 free full-text archive
Gunsalus KC, Yueh WC, MacMenamin P, Piano F
 
mRNA capping enzyme requirement for Caenorhabditis elegans viability.
J Biol Chem   (2003 Apr 18);   PMID: 12576475
Srinivasan P, Piano F, Shatkin AJ
 
Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans.
Curr Biol   (2002 Nov 19);   PMID: 12445391
Piano F, Schetter AJ, Morton DG, Gunsalus KC, Reinke V, Kim SK, Kemphues KJ
 
Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline.
Curr Biol   (2002 Nov 19);   PMID: 12445390
Walhout AJ, Reboul J, Shtanko O, Bertin N, Vaglio P, Ge H, Lee H, Doucette-Stamm L, Gunsalus KC, Schetter AJ, Morton DG, Kemphues KJ, Reinke V, Kim SK, Piano F, Vidal M
 
Updated on 04/05/2014