David J. J. Gresham

Assistant Professor of Biology

Ph.D. 2001 (Human Genetics), Edith Cowan University; B.S. 1997 (Biochemistry), McGill.

Office Address: 

New York University
Department of Biology
Center for Genomics and Systems Biology
12 Waverly Place, Room 203
New York, NY 10003

Phone: 

(212) 998-3879

Fax: 

(212) 995-4015

Lab Homepage: 

http://greshamlab.bio.nyu.edu

Areas of Research/Interest: 

Genomics of adaptive evolution, growth-rate regulation and post-transcriptional gene regulation.

List of Publications from Pubmed

List of citations from Google Scholar

Research:

Our aim is to understand the structure and behavior of the genetic networks that interpret the external environment of the cell. We use the budding yeast to study these networks using a combination of genetic, cell biology and genomic/ computational approaches. Our research addresses three fundamental questions:

1.) What are the pathways, dynamics and principles of adaptive evolution in response to environmental conditions? We perform evolution experiments over hundreds of generations in defined environments using chemostat (continuous) cultures. We study the multigenic basis of evolved quantitative phenotypes to understand the evolutionary trajectories of fitness landscapes and how genes interact to produce quantitative variation.

2.) How does post-transcriptional regulation of gene expression facilitate response to environmental conditions? The response of biological networks to dynamic environments requires processes that occur on very short timescales. The fastest means of altering transcriptional programs is through the degradation or stabilization of pre-existing transcripts. We study the mechanisms that regulate the fate of RNAs in response to environmental signals.

3.) What is the high-resolution structure of genetic interaction networks? To build a map of genetic interactions we employ high throughput suppressor screens using conditional lethal alleles. We use forward and reverse genetic approaches to explore a large fraction of sequence space allowing us to identify both those genes (and their products) that interact and the sequence specificity of those interactions. Our ultimate aim is to infer the rules that govern the interaction and co-evolution of genes.

Selected Works:

Molecular specificity, convergence and constraint shape adaptive evolution in nutrient-poor environments.
PLoS Genet   (2014 Jan);  PMC3886903 free full-text archive
Hong J, Gresham D
 
Design and analysis of Bar-seq experiments.
G3 (Bethesda)   (2014 Jan);  PMC3887526 free full-text archive
Robinson DG, Chen W, Storey JD, Gresham D
 
The use of chemostats in microbial systems biology.
J Vis Exp   (2013);   PMID: 24145466
Ziv N, Brandt NJ, Gresham D
 
Genetic and nongenetic determinants of cell growth variation assessed by high-throughput microscopy.
Mol Biol Evol   (2013 Dec);  PMC3840306 free full-text archive
Ziv N, Siegal ML, Gresham D
 
The details in the distributions: why and how to study phenotypic variability.
Curr Opin Biotechnol   (2013 Aug);  PMC3732567 free full-text archive
Geiler-Samerotte KA, Bauer CR, Li S, Ziv N, Gresham D, Siegal ML
 
Amino acid transporter genes are essential for FLO11-dependent and FLO11-independent biofilm formation and invasive growth in Saccharomyces cerevisiae.
PLoS One   (2012);  PMC3406018 free full-text archive
Torbensen R, Moller HD, Gresham D, Alizadeh S, Ochmann D, Boles E, Regenberg B
 
High-Resolution SNP/CGH Microarrays Reveal the Accumulation of Loss of Heterozygosity in Commonly Used Candida albicans Strains.
G3 (Bethesda)   (2011 Dec);  PMC3276171 free full-text archive
Abbey D, Hickman M, Gresham D, Berman J
 
System-level analysis of genes and functions affecting survival during nutrient starvation in Saccharomyces cerevisiae.
Genetics   (2011 Jan);  PMC3018308 free full-text archive
Gresham D, Boer VM, Caudy A, Ziv N, Brandt NJ, Storey JD, Botstein D
 
Adaptation to diverse nitrogen-limited environments by deletion or extrachromosomal element formation of the GAP1 locus.
Proc Natl Acad Sci U S A   (2010 Oct 26);  PMC2972935 free full-text archive
Gresham D, Usaite R, Germann SM, Lisby M, Botstein D, Regenberg B
 
Dissection of genetically complex traits with extremely large pools of yeast segregants.
Nature   (2010 Apr 15);  PMC2862354 free full-text archive
Ehrenreich IM, Torabi N, Jia Y, Kent J, Martis S, Shapiro JA, Gresham D, Caudy AA, Kruglyak L
 
Optimized detection of sequence variation in heterozygous genomes using DNA microarrays with isothermal-melting probes.
Proc Natl Acad Sci U S A   (2010 Jan 26);  PMC2824413 free full-text archive
Gresham D, Curry B, Ward A, Gordon DB, Brizuela L, Kruglyak L, Botstein D
 
A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds.
Nat Biotechnol   (2009 Apr);  PMC3856559 free full-text archive
Ho CH, Magtanong L, Barker SL, Gresham D, Nishimura S, Natarajan P, Koh JL, Porter J, Gray CA, Andersen RJ, Giaever G, Nislow C, Andrews B, Botstein D, Graham TR, Yoshida M, Boone C
 
Predicting cellular growth from gene expression signatures.
PLoS Comput Biol   (2009 Jan);  PMC2599889 free full-text archive
Airoldi EM, Huttenhower C, Gresham D, Lu C, Caudy AA, Dunham MJ, Broach JR, Botstein D, Troyanskaya OG
 
The repertoire and dynamics of evolutionary adaptations to controlled nutrient-limited environments in yeast.
PLoS Genet   (2008 Dec);  PMC2586090 free full-text archive
Gresham D, Desai MM, Tucker CM, Jenq HT, Pai DA, Ward A, DeSevo CG, Botstein D, Dunham MJ
 
Coordination of growth rate, cell cycle, stress response, and metabolic activity in yeast.
Mol Biol Cell   (2008 Jan);  PMC2174172 free full-text archive
Brauer MJ, Huttenhower C, Airoldi EM, Rosenstein R, Matese JC, Gresham D, Boer VM, Troyanskaya OG, Botstein D
 
Genome-wide analysis of nucleotide-level variation in commonly used Saccharomyces cerevisiae strains.
PLoS One   (2007);  PMC1829191 free full-text archive
Schacherer J, Ruderfer DM, Gresham D, Dolinski K, Botstein D, Kruglyak L
 
Global mapping of transposon location.
PLoS Genet   (2006 Dec 15);  PMC1698948 free full-text archive
Gabriel A, Dapprich J, Kunkel M, Gresham D, Pratt SC, Dunham MJ
 
Accumulation of recessive lethal mutations in Saccharomyces cerevisiae mlh1 mismatch repair mutants is not associated with gross chromosomal rearrangements.
Genetics   (2006 Sep);  PMC1569777 free full-text archive
Heck JA, Gresham D, Botstein D, Alani E
 
Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray.
Science   (2006 Mar 31);   PMID: 16527929
Gresham D, Ruderfer DM, Pratt SC, Schacherer J, Dunham MJ, Botstein D, Kruglyak L
 
Updated on 09/05/2014